LOCUS       sequence-161215-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector for inducible knockdown of human SMAD4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: TET-pLKO.1 PURO shSMAD4 #2
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(46..140)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    46..64
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    74..92
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(110..129)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(429..473)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aactggatta aactgcagtt taatccagtt tctgtctgcc cggtgtctct atcactgata
       61 gggaacttat aagtctctat cactgatagg gatttcacgt ttatggtgat ttcccagaac
      121 acatagcgac atgcaaatat tgcggccgct cgaggctagt ctcgtgatcg ataccgtcga
      181 gatccgttca ctaatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttctatt
      241 ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa
      301 tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct taaacctacc
      361 aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac
      421 caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc
      481 tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg
      541 tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta
      601 gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg
      661 agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg
      721 ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc
      781 tgctgctgca ctataccaga caataattgt ctggcctgta c
//
