LOCUS       sequence-161216-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 and contains guide RNA expression cassette with 
            BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 
            marker for yeast transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  TITLE     Patricia Wittkopp yeast CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pML104-KanMx4
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      344..363
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     terminator      complement(481..668)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     589..613
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(696..716)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aaagataatg tatgattatg ctttcactca tatttataca gaaacttgat gttttctttc
       61 gagtatatac aaggtgatta catgtacgtt tgaagtacaa ctctagattt tgtagtgccc
      121 tcttgggcta gcggtaaagg tgcgcatttt ttcacaccct acaatgttct gttcaaaaga
      181 ttttggtcaa acgctgtaga agtgaaagtt ggtgcgcatg tttcggcgtt cgaaacttct
      241 ccgcagtgaa agataaatga tcagaatcga tgcatttaaa tagcaagtta aaataaggct
      301 agtccgttat caacttgaaa aagtggcacc gagtcggtgg tgcttttttt gttttttatg
      361 tcttcgagtc atgtaattag ttataagcat gtgagctaag acactgtaat tgccaatcta
      421 aacgatacca cggccgctct agagaaatgg ggagcgattt gcaggcattt gctcggcatg
      481 ccggtagagg tgtggtcaat aagagcgacc tcatgctata cctgagaaag caacctgacc
      541 tacaggaaag agttactcaa gaataagaat tttcgtttta aaacctaaga gtcactttaa
      601 aatttgtata cacttatttt ttttataact tatttaataa taaaaatcat aaatcataag
      661 aaattcgcct cgagagtcta ggatccggaa ctacctacct tgcgcttttt cttgggagct
      721 ccctcatccc ctccgagctg tgagaggtca atccgagttt cgtaaagtcc ggtgatgctc
      781 tggtggatga gggtggcgtc cagcacttct ttggtggagg tatagcgttt ccggtcgatg
      841 gtggtgtcga agtacttgaa tgcggcaggg gcaccaagat tggtgagggt gaacaggtgg
      901 atgatattct cagcc
//
