LOCUS       sequence-161217-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 and contains guide RNA expression cassette with 
            BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 
            marker for yeast transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  TITLE     Patricia Wittkopp yeast CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pML104-KanMx4
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        161..504
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(572..591)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttcatcattt tttttttatt cttttttttg atttcggttt ccttgaaatt tttttgattc
       61 ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat tggtatatat
      121 acgcaagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac
      181 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
      241 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
      301 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac
      361 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
      421 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
      481 catcacatcc gaacataaac aaccatgggt aaggaaaaga ctcacgtttc gaggccgcga
      541 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
      601 caatcaggtg cgacaatcta tcgattgtac gggaagcccg atgcgccaga gttgtttctg
      661 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
      721 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
      781 tggttactca ccactgcgat ccccggcaaa acagcattcc aggtattaga agaatatcct
      841 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
      901 cctgtttgta attgtccttt
//
