LOCUS       sequence-161218-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 and contains guide RNA expression cassette with 
            BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 
            marker for yeast transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     Patricia Wittkopp yeast CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pML104-NatMx3
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..277
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     terminator      359..378
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     terminator      complement(496..683)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     604..628
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(711..731)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgcggacaat ctttgaaaga taatgtatga ttatgctttc actcatattt atacagaaac
       61 ttgatgtttt ctttcgagta tatacaaggt gattacatgt acgtttgaag tacaactcta
      121 gattttgtag tgccctcttg ggctagcggt aaaggtgcgc attttttcac accctacaat
      181 gttctgttca aaagattttg gtcaaacgct gtagaagtga aagttggtgc gcatgtttcg
      241 gcgttcgaaa cttctccgca gtgaaagata aatgatcaga atcgatgcat ttaaatagca
      301 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt
      361 tttttgtttt ttatgtcttc gagtcatgta attagttata agcatgtgag ctaagacact
      421 gtaattgcca atctaaacga taccacggcc gctctagaga aatggggagc gatttgcagg
      481 catttgctcg gcatgccggt agaggtgtgg tcaataagag cgacctcatg ctatacctga
      541 gaaagcaacc tgacctacag gaaagagtta ctcaagaata agaattttcg ttttaaaacc
      601 taagagtcac tttaaaattt gtatacactt atttttttta taacttattt aataataaaa
      661 atcataaatc ataagaaatt cgcctcgaga gtctaggatc cggaactacc taccttgcgc
      721 tttttcttgg gagctccctc atcccctccg agctgtgaga ggtcaatccg agtttcgtaa
      781 agtccggtga tgctctggtg gatgagggtg gcgtccagca cttctttggt ggaggtatag
      841 cg
//
