LOCUS       sequence-161224-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 and contains guide RNA expression cassette with 
            BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 
            marker for yeast transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  TITLE     Patricia Wittkopp yeast CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pML104-NatMx3
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        161..504
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 ttcatcattt tttttttatt cttttttttg atttcggttt ccttgaaatt tttttgattc
       61 ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat tggtatatat
      121 acgcatgctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac
      181 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
      241 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
      301 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac
      361 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
      421 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
      481 catcacatcc gaacataaac aaccatgggt accactcttg acgacacggc ttaccggtac
      541 cgcaccagtg tcccggggga cgccgaggcc atcgaggcac tggatgggtc cttcaccacc
      601 gacaccgtct tccgcgtcac cgccaccggg gacggcttca ccctgcggga ggtgccggtg
      661 gacccgcccc tgaccaaggt gttccccgac gacgaatcgg acgacgaatc ggacgacggg
      721 gaggacggcg acccggactc ccggacgttc gtcgcgtacg gggacgacgg cgacctggcg
      781 ggcttcgtgg tcgtctcgta ctccggctgg aaccgccggc tgaccgtcga ggacatcgag
      841 gtcgccccgg agcaccgggg
//
