LOCUS       sequence-161226-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3' entry vector containing HA epitope fused to C-terminus and 
            mCherry fused to N-terminus of FRB without pA for rapamycin/rapalog 
            induced dimerization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-mCherry-FRB-HA no-pA
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..128
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(135..161)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(168..437)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     primer_bind     complement(518..541)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(716..735)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcgcggccgc gttaacgcta ccatggagct ccaaataatg attttatttt gactgatagt
       61 gacctgttcg ttgcaacaaa ttgataagca atgctttttt ataatgccaa ctttgtataa
      121 taaagttgct atgctgcgta gtctggtacg tcgtacggat agtcgacctg ctttgagatt
      181 cgtcggaaca catgataata gaggtcccag gcttgggtga ggtccttgac attccctgat
      241 ttcatgtact tcctgcacca ctcttgggcc tccattaaat ctcgaccata ggcctgatta
      301 aaggatgttt ccttcagagt ctgggggccc cgttccatca tagcatgcaa gggctccagc
      361 acctcaaaca tgcctttcac gttcctttcc ccaaagtaca aacgagatgc ctcttccagg
      421 ccttcatgcc acatctcgac cttgtacagc tcgtccatgc cgccggtgga gtggcggccc
      481 tcggcgcgct cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc
      541 ttgatgttga cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc
      601 ttgacctcgg cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc
      661 ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc
      721 ttctgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg
      781 aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgc
//
