LOCUS       sequence-161228-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of SMAD4 with 3xFLAG 
            N-terminal tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pSLIK TT 3xFLAG SMAD4 neo
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    148..172
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     ncRNA           complement(211..323)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 aggatcagga cgctcgctgc gcccttcgtc tgacgtggca gcgctcgccg tgaggagggg
       61 ggcgcccgcg ggaggcgcca aaacccggcg cggaggccag atcttgggtg ggttaattaa
      121 ccatctttat ggctcgagat gttatcaacc actttgtaca agaaagctgg gtctagatat
      181 ctcgaaaacg cattagtctt ccaattgaaa aaagtgattt aatttatacc attttaattc
      241 agctttgtaa aaatgtatca aagagatagc aaggtattca gttttagtaa acaagataat
      301 tgctcctaaa gtagcccctt gaattctcag tctaaaggtt gtgggtctgc aatcggcatg
      361 gtatgaagta cttcgtctag gagctggagg gcccggtgta agtgaatttc aatccagcaa
      421 ggtgtttctt tgatgctctg tcttgggtaa tccggtcccc agcctttcac aaaactcatc
      481 ctgagtatgc ataagcgacg aaggtcatca acaccaattc cagcagcagc tgacagactg
      541 atagctggag ctattccacc tactgatcct gggccaggga tgtttcctgc cacggctgct
      601 gcctgggcag ctgctgcagc ttgtgcagta gccgcctgct gctgcatctg tcgatgacac
      661 tgacgcaaat caaagacctt tatatatgca cttgggtaga tcttatgaac agcatctcca
      721 ggtgcacgcc cagcttctct gtctaagtag taactctgta caaagaccgc gtggtcacta
      781 aggcacctga cccaaacatc accttcacct ttacattcca actgcacacc tttgcctatg
      841 tgcaaccttg ctctctca
//
