LOCUS       sequence-161231-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3' entry vector containing HA epitope fused to C-terminus and 
            mCherry fused to N-terminus of FRB without pA for rapamycin/rapalog 
            induced dimerization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-mCherry-FRB-HA no-pA
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..169)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(314..332)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     582..601
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gagctcgaat tctacaggtc actaatacca tctaagtagt tggttcatag tgactgcata
       61 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
      121 atatttatat cattttacgt ttctcgttca actttcttgt acaaagtggc ggtgagcaag
      181 ggcgaggagg acaacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      241 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      301 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      361 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
      421 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      481 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
      541 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
      601 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
      661 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgccga ggtcaagacc
      721 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagctg
      781 gacatcacct cc
//
