LOCUS       sequence-161233-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for integration at yeast URA3 locus, expression 
            from the GPD promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  TITLE     Nemhauser plasmids
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGP6G-ccdB
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..81
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     65..81
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    89..213
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(238..243,244..261,262..268)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(388..407)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aattctactt ttatagttag tctttttttt agttttaaaa caccagaact tagtttcgac
       61 ggattctaga actagtggat cccccatcac aagtttgtac aaaaaagctg aacgagaaac
      121 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
      181 atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt
      241 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt
      301 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
      361 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
      421 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
      481 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
      541 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
      601 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      661 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      721 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      781 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccc
//
