LOCUS       sequence-161246-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of human GDF11 with 
            C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pLX302 GDF11-V5 puro
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..98)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    100..124
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atccagaggt tgattgtcga cttaacgcgc caccggttag cgctagctca ttactacgta
       61 gaatcgagac cgaggagagg gttagggata ggcttaccaa ccactttgta caagaaagct
      121 gggcccatga ggcctgaaga gcagccacag cgatccacca ccatgccagg gatcttgccg
      181 tagataatct gctgcttgtc attgaagtag agcatgttga ttggggacat cttggtgggg
      241 gtacaacagg gcccagcaga gcctcttgga ttggcctgct gcaccaaatg ggtatgcgga
      301 tatttttgca tgaacatgta ctcgcactgg ccggagcagt agttggcctt gtagcgctta
      361 ggtgcgatga tccagtccca gccgaaagcc tcaaagtcca ctgtgagggg atatcggcag
      421 cagcgggact cgcttgagtg ctcgtcgcag tccagaccca ggttccgccg ggaacgtttt
      481 gtgttctcta ggactcgaag ctccatgaat ggatgcagcc cctcggctcc cggccccagg
      541 gaggtgacag ccaggtctgt gccactggga tcaaaggcgt tgatctcgat gccccagttg
      601 ctctgtggct ggcggaacca gctgtgtagc acttgcttga agtcgatgct ctgccaatgg
      661 cctgagcgtg agtgcagctc aatcttcagt gagcggatac ggatgtgacg ccggcctccg
      721 ccccctcccc ctgcggtccc ttccccagtt agg
//
