LOCUS       sequence-161260-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent coding joint substrate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Neal JA, Xu Y, Abe M, Hendrickson E, Meek K
  TITLE     Restoration of ATM Expression in DNA-PKcs-Deficient Cells Inhibits 
            Signal End Joining.
  JOURNAL   J Immunol. 2016 Apr 1;196(7):3032-42. doi: 10.4049/jimmunol.1501654.
            Epub 2016 Feb 26.
  PUBMED    26921311
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501654"; journalName: "J Immunol"; date: 
            "2016-04-1- 1"; volume: "196"; issue: "7"; pages: "3032-42"
COMMENT     Sequence Label: 290 RFP/CFP
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(98..775)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGALKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(160..183)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(358..377)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     575..595
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     627..645
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtcccac agtggtagta ctccactgtc
       61 tggctgtaca aaaaccctcg ggatcctctc atcgatgcta caggaacagg tggtggcggc
      121 cctcggcgcg ctcgtactgc tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
      181 gcttggagtc cacgtagtag tagccgggca gctgcacggg cttcttggcc atgtagatgg
      241 acttgaactc caccaggtag tggccgccgt ccttcagctt cagggccttg tggatctcgc
      301 ccttcagcgc gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag cccatagtct
      361 tcttctgcat tacggggccg tcggagggga agttcacgcc gatgaacttc accttgtaga
      421 tgaagcttcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga
      481 agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcttg tagtcgggga
      541 tgtcggcggg gtgcttcacg tacaccttgg agccgtactg gaactggggg gacaggatgt
      601 cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag cttggcggtc tgggtgccct
      661 cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct
      721 ccatgcgcac cttgaagcgc atgaactcct tgatgacgtc ctcggaggag gccatggtat
      781 cgatgctaca ggaacaggtg gtgg
//
