LOCUS       sequence-161268-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent Isce joint substrate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Neal JA, Xu Y, Abe M, Hendrickson E, Meek K
  TITLE     Restoration of ATM Expression in DNA-PKcs-Deficient Cells Inhibits 
            Signal End Joining.
  JOURNAL   J Immunol. 2016 Apr 1;196(7):3032-42. doi: 10.4049/jimmunol.1501654.
            Epub 2016 Feb 26.
  PUBMED    26921311
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501654"; journalName: "J Immunol"; date: 
            "2016-04-1- 1"; volume: "196"; issue: "7"; pages: "3032-42"
COMMENT     Sequence Label: isce rfp/cfp
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(141..854,855..857,858..860))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(186..207)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     535..554
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     794..815
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      857..866
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
       61 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
      121 gatctagagt cgcggccgct ttacttgtac agctcgtcca tgccgagagt gatcccggcg
      181 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
      241 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      301 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
      361 ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtggctg
      421 atgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      481 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      541 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
      601 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccc
      661 caggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
      721 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
      781 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
      841 tcctcgccct tgctcaccat ggtggcgacc ggtatcagtt acgctaggga taacagggta
      901 atataggatc ctctcatcga tgctacagga acaggtggtg gcggccctcg gcgcgctcgt
      961 actgctcca
//
