LOCUS       sequence-161269-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent Isce joint substrate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Neal JA, Xu Y, Abe M, Hendrickson E, Meek K
  TITLE     Restoration of ATM Expression in DNA-PKcs-Deficient Cells Inhibits 
            Signal End Joining.
  JOURNAL   J Immunol. 2016 Apr 1;196(7):3032-42. doi: 10.4049/jimmunol.1501654.
            Epub 2016 Feb 26.
  PUBMED    26921311
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501654"; journalName: "J Immunol"; date: 
            "2016-04-1- 1"; volume: "196"; issue: "7"; pages: "3032-42"
COMMENT     Sequence Label: isce rfp/cfp
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(90..767)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGALKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(152..175)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(350..369)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     567..587
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     619..637
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt atcagttacg ctagggataa
       61 cagggtaata taggatcctc tcatcgatgc tacaggaaca ggtggtggcg gccctcggcg
      121 cgctcgtact gctccacgat ggtgtagtcc tcgttgtggg aggtgatgtc cagcttggag
      181 tccacgtagt agtagccggg cagctgcacg ggcttcttgg ccatgtagat ggacttgaac
      241 tccaccaggt agtggccgcc gtccttcagc ttcagggcct tgtggatctc gcccttcagc
      301 gcgccgtcgc gggggtacag gcgctcggtg gaggcctccc agcccatagt cttcttctgc
      361 attacggggc cgtcggaggg gaagttcacg ccgatgaact tcaccttgta gatgaagctt
      421 ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc
      481 acgcgctccc acttgaagcc ctcggggaag gacagcttct tgtagtcggg gatgtcggcg
      541 gggtgcttca cgtacacctt ggagccgtac tggaactggg gggacaggat gtcccaggcg
      601 aagggcaggg ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg
      661 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgcgc
      721 accttgaagc gcatgaactc cttgatgacg tcctcggagg aggccatggt atcgatgaga
      781 gtcgactata ttaccctgtt atccctagcg taacttcgac ctgcagccca agcttgggct
      841 agcggatctg acggttcact aaaccagctc tgc
//
