LOCUS       sequence-161278-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid contains LIC cassette upstream of an in-frame copy of YFP 
            and DHFR-TS selectable marker cassette for endogenous tagging on the
            gene locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Huynh MH, Carruthers VB
  TITLE     Tagging of endogenous genes in a Toxoplasma gondii strain lacking 
            Ku80.
  JOURNAL   Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 
            2009 Feb 13.
  PUBMED    19218426
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/EC.00358-08"; journalName: "Eukaryot Cell"; date: 
            "2009-04"; volume: "8"; issue: "4"; pages: "530-9"
COMMENT     Sequence Label: pYFP.LIC.DHFR
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cggccgctct agaactagtg gatcgatccc ccgggctgca ggaattccat cctgcaagtg
       61 catagaagga aagttgtctg ctgtcgtggg cagacagcaa cagtccagca ctctagcggc
      121 atacagaacg ataacgcatt cacgagtgga tacacgcaca tctgcgtcac ccgcaactcg
      181 ctttcgttct gattgacaaa aagaaaacaa ggcgaggtga gactgtgtga aatgccacat
      241 gaagagtcat cccttttctt cgataaagga cacaggggtc tctggcaccc cctcgtcagc
      301 tctctccgac ccgaggcact ctccctgatc cctccgaaaa gagaggaaaa cgagagacgg
      361 gcagcttctg tatttccgct agacagccat ctccatctgg attcgtccgt gcgggacgta
      421 gcccacgacc tcaaaatcct cggcggtgaa atcgtcgatt tccttgatgc gttccttgtt
      481 gaggatgttc acaatgggga acggtctcgg ttctctccgc agctgctctt ttaaagcctc
      541 gacatggttc gtgtagacat gcgtgttccc catgaagtga atgaactcct taggttttag
      601 gttgcagacg tgtgcaacca tgagcgtcaa aagcgaatag gaagcgatgt tgaaggggac
      661 gccgaggccg acatcgcacg accgctgata catgatgcac gacagctcct tctggtcgtt
      721 cacgtagaac tggcacaaca agtgacaagg cggcagcgcc atttcgtcca gcgctgcagg
      781 attccaggca gtcatgagca tgcgacgatc tgttggattc gttctcagca tctggatcac
      841 attcttcagc tggtcgacgc cctgccctgt gtagtctgtg tgcatgtctt tgtatgccgc
      901 gccgaagtgt ctccactgga agccgtagcc cgggccgatg tctccgacct ctcggtgggg
      961 gagattgcgc gaatcgagga actcgcgtgt cacattcttg
//
