LOCUS       sequence-161281-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3' entry vector containing HA epitope tag fused to C-terminus of FRB
            mutant (W2101F) without pA for rapamycin/rapalog induced 
            dimerization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-FRB(KTF)-HA no-pA
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    32..127
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(134..160)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    433..556
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(627..644)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(627..643)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(636..658)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      707..753
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgcggccgcg ttaacgctac catggagctc caaataatga ttttattttg actgatagtg
       61 acctgttcgt tgcaacaaat tgataagcaa tgctttttta taatgccaac tttgtataat
      121 aaagttgcta tgctgcgtag tctggtacgt cgtacggata gtcgacctgc tttgagattc
      181 gtcggaacac atgataatag aggtcgaagg cttgggtgag gtccttgaca ttccctgatt
      241 tcatgtactt cctgcaccac tcttgggcct ccattaaatc tcgaccatag gcctgattaa
      301 aggatgtttc cttcagagtc tgggggcccc gttccatcat agcatgcaag ggctccagca
      361 cctcaaacat gcctttcacg ttcctttccc caaagtacaa acgagatgcc tcttccaggc
      421 cttcatgcca cgccactttg tacaagaaag ttgaacgaga aacgtaaaat gatataaata
      481 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
      541 atatgcagtc actatgaacc aactacttag atggtattag tgacctgtag aattcgagct
      601 ctagagctgc agggcccgag cttaagactg gccgtcgttt tacaacgtcg tgactgggaa
      661 aacatccatg ctagcgttaa cgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
      721 ggctcagtcg gaagactggg cctttcgttt tatctgttgt ttgtcggtga
//
