LOCUS       sequence-161282-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3' entry vector containing HA epitope tag fused to C-terminus of FRB
            mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog 
            induced dimerization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-FRB(PLF)-HA no-pA
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..169)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             442..468
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(475..570)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(607..626)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(608..626)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(631..647)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(631..647)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(749..768)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gagctcgaat tctacaggtc actaatacca tctaagtagt tggttcatag tgactgcata
       61 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
      121 atatttatat cattttacgt ttctcgttca actttcttgt acaaagtggc gtggcatgaa
      181 ggcctggaag aggcatctcg tttgtacttt ggggaaagga acgtgaaagg catgtttgag
      241 gtgctggagc ccttgcatgc tatgatggaa cggggccccc agactctgaa ggaaacatcc
      301 tttaatcagg cctatggtcg agatttaatg gaggcccaag agtggtgcag gaagtacatg
      361 aaatcaggga atgtcccgga cctcctccaa gccttcgacc tctattatca tgtgttccga
      421 cgaatctcaa agcaggtcga ctatccgtac gacgtaccag actacgcagc atagcaactt
      481 tattatacaa agttggcatt ataaaaaagc attgcttatc aatttgttgc aacgaacagg
      541 tcactatcag tcaaaataaa atcattattt ggagctccat ggtagcgtta acgcggccgc
      601 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
      661 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
      721 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
      781 acgtcgaggc cgcgattaaa
//
