LOCUS       sequence-161283-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3' entry vector containing HA epitope tag fused to C-terminus of FRB
            mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog 
            induced dimerization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-FRB(PLF)-HA no-pA
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..128
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(135..161)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    434..557
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(628..645)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(628..644)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(637..659)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      708..794
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcgcggccgc gttaacgcta ccatggagct ccaaataatg attttatttt gactgatagt
       61 gacctgttcg ttgcaacaaa ttgataagca atgctttttt ataatgccaa ctttgtataa
      121 taaagttgct atgctgcgta gtctggtacg tcgtacggat agtcgacctg ctttgagatt
      181 cgtcggaaca catgataata gaggtcgaag gcttggagga ggtccgggac attccctgat
      241 ttcatgtact tcctgcacca ctcttgggcc tccattaaat ctcgaccata ggcctgatta
      301 aaggatgttt ccttcagagt ctgggggccc cgttccatca tagcatgcaa gggctccagc
      361 acctcaaaca tgcctttcac gttcctttcc ccaaagtaca aacgagatgc ctcttccagg
      421 ccttcatgcc acgccacttt gtacaagaaa gttgaacgag aaacgtaaaa tgatataaat
      481 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
      541 catatgcagt cactatgaac caactactta gatggtatta gtgacctgta gaattcgagc
      601 tctagagctg cagggcccga gcttaagact ggccgtcgtt ttacaacgtc gtgactggga
      661 aaacatccat gctagcgtta acgcgagagt agggaactgc caggcatcaa ataaaacgaa
      721 aggctcagtc ggaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
      781 tgagtaggac aaatccgccg ggagcggatt
//
