LOCUS       sequence-161284-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HA epitope tag fused to C-terminus of human FK506 binding protein 12
            (FKBP12) without pA for rapamycin/rapalog induced dimerization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-FKBP-HA no-pA
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..169)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             172..492
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             172..492
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             499..525
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(532..627)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(664..683)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(665..683)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(688..704)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(688..704)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gagctcgaat tctacaggtc actaatacca tctaagtagt tggttcatag tgactgcata
       61 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
      121 atatttatat cattttacgt ttctcgttca actttcttgt acaaagtggc gggcgtgcag
      181 gtggagacta tctccccagg agacgggcgc accttcccca agcgcggcca gacctgcgtg
      241 gtgcactaca ccgggatgct tgaagatgga aagaaatttg attcctcccg ggacagaaac
      301 aagcccttta agtttatgct aggcaagcag gaggtgatcc gaggctggga agaaggggtt
      361 gcccagatga gtgtgggtca gagagccaaa ctgactatat ctccagatta tgcctatggt
      421 gccactgggc acccaggcat catcccacca catgccactc tcgtcttcga tgtggagctt
      481 ctaaaactgg aagtcgacta tccgtacgac gtaccagact acgcagcata gcaactttat
      541 tatacaaagt tggcattata aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca
      601 ctatcagtca aaataaaatc attatttgga gctccatggt agcgttaacg cggccgcgat
      661 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
      721 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
      781 ctgtctgctt acataaacag taatacaagg g
//
