LOCUS       sequence-161286-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HA epitope tag fused to C-terminus of human FK506 binding protein 12
            (FKBP12) without pA for rapamycin/rapalog induced dimerization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-FKBP-HA no-pA
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..128
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(135..161)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(168..488)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(168..488)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     protein_bind    491..614
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(685..702)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(685..701)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(694..716)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tcgcggccgc gttaacgcta ccatggagct ccaaataatg attttatttt gactgatagt
       61 gacctgttcg ttgcaacaaa ttgataagca atgctttttt ataatgccaa ctttgtataa
      121 taaagttgct atgctgcgta gtctggtacg tcgtacggat agtcgacttc cagttttaga
      181 agctccacat cgaagacgag agtggcatgt ggtgggatga tgcctgggtg cccagtggca
      241 ccataggcat aatctggaga tatagtcagt ttggctctct gacccacact catctgggca
      301 accccttctt cccagcctcg gatcacctcc tgcttgccta gcataaactt aaagggcttg
      361 tttctgtccc gggaggaatc aaatttcttt ccatcttcaa gcatcccggt gtagtgcacc
      421 acgcaggtct ggccgcgctt ggggaaggtg cgcccgtctc ctggggagat agtctccacc
      481 tgcacgcccg ccactttgta caagaaagtt gaacgagaaa cgtaaaatga tataaatatc
      541 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
      601 atgcagtcac tatgaaccaa ctacttagat ggtattagtg acctgtagaa ttcgagctct
      661 agagctgcag ggcccgagct taagactggc cgtcgtttta caacgtcgtg actgggaaaa
      721 catccatgct agcgttaacg cgagagtagg gaactgccag gcatcaaata a
//
