LOCUS       sequence-161288-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid contains LIC cassette upstream of an in-frame copy of YFP 
            and HXGPRT selectable marker cassette for endogenous tagging on the 
            gene locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Huynh MH, Carruthers VB
  TITLE     Tagging of endogenous genes in a Toxoplasma gondii strain lacking 
            Ku80.
  JOURNAL   Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 
            2009 Feb 13.
  PUBMED    19218426
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/EC.00358-08"; journalName: "Eukaryot Cell"; date: 
            "2009-04"; volume: "8"; issue: "4"; pages: "530-9"
COMMENT     Sequence Label: pYFP.LIC.HXGPRT
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..31
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15..31
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gcggtggcgg ccgctctaga actagtggat ccccctccac cgcggtgtca ctgtagcctg
       61 ccagaacact tgtcaaccga ctgtgtccac atttttatgc gcactgactg gcatgaatgg
      121 ccagaggcag gcatcagcaa gtcacgtagg ccaacgcgtg cgcagaaacg ctcaaggctc
      181 gattgtgggt gggggttggt agcattttat cgacctaaac aaggtttaca cttaggtggt
      241 gcggttttac tgatctggac ggattcagcg gtcgcagatt atcgatctgc aaatggtgta
      301 cacttaggtg tcgcggctta tctagttaag ggagcttcgt ggtcggagcc taacaagtca
      361 acagagacgt atcgccaatc gttcgcggtg aagagtcgaa actgacagca catcgtaggg
      421 aaactgagag ggtgctcctt tctctccgtc gtttgcgctg caccatcctg caagtgcata
      481 gaaggaaagt tgtctgctgt cgtgggcaga cagcaacagt ccagcactct agcggcatac
      541 agaacgataa cgcattcacg agtggataca cgcacatctg cgtcacccgc aactcgcttt
      601 cgttctgatt gacaaaaaga aaacaaggcg aggtgagact gtgtgaaatg ccacatgaag
      661 agtcatccct tttcttcgat aaaggacaca ggggtctctg gcaccccctc gtcagctctc
      721 tccgacccga ggcactctcc ctgatccctc cgaaaagaga ggaaaacgag agacgggcag
      781 cttctgtggg ctatgcaggg tttacttctc gaactttttg cgagcggcgt cgctcaggac
      841 ggcgacgtgg tcgaagtcgc ggaacat
//
