LOCUS       sequence-161289-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3' entry vector containing streptavidin binding protein (SBP) 
            followed by a TEV cleavage site and 2 protein G copies without pA 
            for tandem affinity purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-SGTAP no-pA
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(45..168)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             171..284
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             291..311
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             318..338
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(714..734)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 agctcgaatt ctacaggtca ctaataccat ctaagtagtt ggttcatagt gactgcatat
       61 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
      121 tatttatatc attttacgtt tctcgttcaa ctttcttgta caaagtggcg atggacgaga
      181 agaccaccgg gtggcggggc ggccacgttg tggagggtct cgctggcgag ctggagcagc
      241 tcagggcccg cttggagcac catccccagg ggcaacgcga gcctatcgat gaaaatctgt
      301 acttccaggg gatcgatgag aacctgtact ttcagggggg tagtaccccg gccgtgacga
      361 cctacaagct ggtgatcaac ggcaagaccc tgaagggcga gaccacaacc aaggccgtgg
      421 acgctgaaac cgcagagaag gccttcaagc agtacgccaa cgacaatggc gtggacggag
      481 tgtggaccta cgacgatgcc accaagacct tcaccgtgac cgaggttaac acccccgcag
      541 tcaccacata caaactggtg atcaacggaa aaacgctgaa gggcgaaact acgaccaagg
      601 ctgtggacgc ggagacggcc gaaaaggcgt tcaaacagta tgccaacgat aacggtgtag
      661 acggcgtctg gacatatgac gacgcgacaa agactttcac tgtcactgaa tagcaacttt
      721 attatacaaa gttggcatta taaa
//
