LOCUS       sequence-161290-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3' entry vector containing streptavidin binding protein (SBP) 
            followed by a TEV cleavage site and 2 protein G copies without pA 
            for tandem affinity purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-SGTAP no-pA
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    32..127
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(503..523)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(530..550)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(557..670)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    672..696
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgcggccgcg ttaacgctac catggagctc caaataatga ttttattttg actgatagtg
       61 acctgttcgt tgcaacaaat tgataagcaa tgctttttta taatgccaac tttgtataat
      121 aaagttgcta ttcagtgaca gtgaaagtct ttgtcgcgtc gtcatatgtc cagacgccgt
      181 ctacaccgtt atcgttggca tactgtttga acgccttttc ggccgtctcc gcgtccacag
      241 ccttggtcgt agtttcgccc ttcagcgttt ttccgttgat caccagtttg tatgtggtga
      301 ctgcgggggt gttaacctcg gtcacggtga aggtcttggt ggcatcgtcg taggtccaca
      361 ctccgtccac gccattgtcg ttggcgtact gcttgaaggc cttctctgcg gtttcagcgt
      421 ccacggcctt ggttgtggtc tcgcccttca gggtcttgcc gttgatcacc agcttgtagg
      481 tcgtcacggc cggggtacta cccccctgaa agtacaggtt ctcatcgatc ccctggaagt
      541 acagattttc atcgataggc tcgcgttgcc cctggggatg gtgctccaag cgggccctga
      601 gctgctccag ctcgccagcg agaccctcca caacgtggcc gccccgccac ccggtggtct
      661 tctcgtccat cgccactttg tacaagaaag ttgaacgaga aacgtaaaat gatataaata
      721 tcaatatatt aaattagatt ttgcataa
//
