LOCUS       sequence-161299-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid contains LIC cassette upstream of an in-frame copy of 
            mCherry and HXGPRT selectable marker cassette for endogenous tagging
            on the gene locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Huynh MH, Carruthers VB
  TITLE     Tagging of endogenous genes in a Toxoplasma gondii strain lacking 
            Ku80.
  JOURNAL   Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 
            2009 Feb 13.
  PUBMED    19218426
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/EC.00358-08"; journalName: "Eukaryot Cell"; date: 
            "2009-04"; volume: "8"; issue: "4"; pages: "530-9"
COMMENT     Sequence Label: pmCherry.LIC.HXGPRT
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..33
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17..33
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ccgcggtggc ggccgctcta gaactagtgg atccccctcc accgcggtgt cactgtagcc
       61 tgccagaaca cttgtcaacc gactgtgtcc acatttttat gcgcactgac tggcatgaat
      121 ggccagaggc aggcatcagc aagtcacgta ggccaacgcg tgcgcagaaa cgctcaaggc
      181 tcgattgtgg gtgggggttg gtagcatttt atcgacctaa acaaggttta cacttaggtg
      241 gtgcggtttt actgatctgg acggattcag cggtcgcaga ttatcgatct gcaaatggtg
      301 tacacttagg tgtcgcggct tatctagtta agggagcttc gtggtcggag cctaacaagt
      361 caacagagac gtatcgccaa tcgttcgcgg tgaagagtcg aaactgacag cacatcgtag
      421 ggaaactgag agggtgctcc tttctctccg tcgtttgcgc tgcaccatcc tgcaagtgca
      481 tagaaggaaa gttgtctgct gtcgtgggca gacagcaaca gtccagcact ctagcggcat
      541 acagaacgat aacgcattca cgagtggata cacgcacatc tgcgtcaccc gcaactcgct
      601 ttcgttctga ttgacaaaaa gaaaacaagg cgaggtgaga ctgtgtgaaa tgccacatga
      661 agagtcatcc cttttcttcg ataaaggaca caggggtctc tggcaccccc tcgtcagctc
      721 tctccgaccc gaggcactct ccctgatccc tccgaaaaga gaggaaaacg agagacgggc
      781 agcttctgtg ggctatgcag ggtttacttc tcgaactttt tgcgagcggc gtcgctcagg
      841 acggcgacgt ggtcgaagtc gcggaacatc tcgttgaagt cgtagcagca accaacgatc
      901 cagacgtctt caatgctgaa gccgacgaag tcgcccttca agctgttgga gcgatctgtg
      961 cgcttctcga cgagggtggc gattctcatc gacttgggac cgacggcttt caggcgctca
     1021 ccgaactcgg tgagggtgaa accggtgt
//
