LOCUS       sequence-161301-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector for inducible knockdown of human SMAD4 
            (cross reacts with mouse Smad4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: TET-pLKO.1 PURO shSMAD4 #1
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(57..151)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    57..75
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    85..103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(121..140)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(440..484)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aagccagcta cttaccatca taactgcagt tatgatggta agtagctggc ccggtgtctc
       61 tatcactgat agggaactta taagtctcta tcactgatag ggatttcacg tttatggtga
      121 tttcccagaa cacatagcga catgcaaata ttgcggccgc tcgaggctag tctcgtgatc
      181 gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
      241 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
      301 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
      361 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
      421 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
      481 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
      541 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
      601 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
      661 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
      721 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
      781 gcaa
//
