LOCUS       sequence-161302-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector for IAA1 with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA1_no stop
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(609..708)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(725..744)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(726..744)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(749..765)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(749..765)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg
       61 cttttttata atgccaactt tgtacaaaaa agcaggcttc aaaatggaag tcaccaatgg
      121 gcttaacctt aaggacacag agcttcgttt gggattaccc ggagcacaag aagaacaaca
      181 actagaactt tcttgcgtca gaagcaacaa caagcgcaag aacaacgact caacagaaga
      241 atctgctcct cctcctgcaa aaacacaaat cgttggatgg cctccagtga gatctaaccg
      301 taagaacaac aacaacaaaa acgtgagtta tgtgaaagtg agtatggacg gagctccata
      361 tctccgtaag atagatctca agatgtacaa aaactatcca gagcttctca aagcactaga
      421 gaacatgttc aagttcacag taggtgaata ttccgagaga gaaggctaca aaggatctgg
      481 atttgtacct acttatgaag acaaagatgg agattggatg ttggtcggtg atgttccatg
      541 ggacatgttc tcttcatctt gtcaaaaact cagaatcatg aaaggatccg aagctcctac
      601 tgccttacac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
      661 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
      721 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctg
//
