LOCUS       sequence-161303-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid contains LIC cassette upstream of an in-frame copy of 
            tdTomato and HXGPRT selectable marker cassette for endogenous 
            tagging on the gene locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Huynh MH, Carruthers VB
  TITLE     Tagging of endogenous genes in a Toxoplasma gondii strain lacking 
            Ku80.
  JOURNAL   Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 
            2009 Feb 13.
  PUBMED    19218426
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/EC.00358-08"; journalName: "Eukaryot Cell"; date: 
            "2009-04"; volume: "8"; issue: "4"; pages: "530-9"
COMMENT     Sequence Label: ptdTomato.LIC.HXGPRT
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..25
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9..25
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gcggccgctc tagaactagt ggatccccct ccaccgcggt gtcactgtag cctgccagaa
       61 cacttgtcaa ccgactgtgt ccacattttt atgcgcactg actggcatga atggccagag
      121 gcaggcatca gcaagtcacg taggccaacg cgtgcgcaga aacgctcaag gctcgattgt
      181 gggtgggggt tggtagcatt ttatcgacct aaacaaggtt tacacttagg tggtgcggtt
      241 ttactgatct ggacggattc agcggtcgca gattatcgat ctgcaaatgg tgtacactta
      301 ggtgtcgcgg cttatctagt taagggagct tcgtggtcgg agcctaacaa gtcaacagag
      361 acgtatcgcc aatcgttcgc ggtgaagagt cgaaactgac agcacatcgt agggaaactg
      421 agagggtgct cctttctctc cgtcgtttgc gctgcaccat cctgcaagtg catagaagga
      481 aagttgtctg ctgtcgtggg cagacagcaa cagtccagca ctctagcggc atacagaacg
      541 ataacgcatt cacgagtgga tacacgcaca tctgcgtcac ccgcaactcg ctttcgttct
      601 gattgacaaa aagaaaacaa ggcgaggtga gactgtgtga aatgccacat gaagagtcat
      661 cccttttctt cgataaagga cacaggggtc tctggcaccc cctcgtcagc tctctccgac
      721 ccgaggcact ctccctgatc cctccgaaaa gagaggaaaa cgagagacgg gcagcttctg
      781 tgggctatgc agggtttact tctcgaactt tttgcgagcg gcgtcgctca ggacggcgac
      841 gtggtcgaag tcgcggaaca tctcgttgaa gtcgtagcag caaccaacga tccagacgtc
      901 ttcaatgctg aagcc
//
