LOCUS       sequence-161308-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Calreticulin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Del Cid N, Jeffery E, Rizvi SM, Stamper E, Peters LR, Brown WC, 
            Provoda C, Raghavan M
  TITLE     Modes of calreticulin recruitment to the major histocompatibility 
            complex class I assembly pathway.
  JOURNAL   J Biol Chem. 2010 Feb 12;285(7):4520-35. doi: 
            10.1074/jbc.M109.085407. Epub 2009 Dec 3.
  PUBMED    19959473
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.085407"; journalName: "J Biol Chem"; date: 
            "2010-02-12- 12"; volume: "285"; issue: "7"; pages: "4520-35"
COMMENT     Sequence Label: pmcsg7-mCRT(WT)
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccaatgct
      121 gccgcagacc ctgccatcta tttcaaagag cagttcttgg acggagatgc ctggaccaac
      181 cgctgggtcg aatccaaaca taagtccgat tttggcaaat ttgtcctcag ttctggcaaa
      241 ttttacgggg acctggagaa ggataaaggg ctgcagacaa gccaagatgc ccgattttac
      301 gcactgtccg ccaaattcga acccttcagc aataagggcc agacactggt ggtacagttc
      361 acggtgaagc atgagcagaa tatcgactgt gggggcggct acgtgaagct gtttccgagt
      421 ggcttggacc agaaggacat gcatggagac tcagaatata acatcatgtt tggtccggac
      481 atctgcggtc ctggcaccaa gaaggttcat gtcatcttta actacaaggg caagaatgtg
      541 ctgatcaaca aggatatccg gtgtaaggat gatgaattca cacacctata cacactgatt
      601 gtgcggccag acaacaccta tgaggtgaaa attgacaaca gccaggtgga gtcaggctcc
      661 ttggaggatg attgggactt tctgccaccc aagaagataa aggaccctga tgctgccaag
      721 ccggaagact gggatgaacg agccaagatc gatgacccca cagattccaa gcctgaggac
      781 tgggacaagc cagagcacat ccctgaccct gatgctaaga agcctgagga ctgggatgaa
      841 gagatggatg gagagtggga accaccagtg attcaaaatc ctgaatacaa gggcgagtgg
      901 aaaccacgtc aaattgacaa cccagattac aagggtacct ggatacaccc
//
