LOCUS       sequence-161313-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  minimal cytomegalovirus immediate early enhancer/promoter (CMVmin) 
            from pcDNA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p5E-CMVmin
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(68..191)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(192..395)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(221..241)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        396..699
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(761..781)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 tcgcggccgc cctgcagctc tagagctcga attctacagg tcactaatac catctaagta
       61 gttggttcat agtgactgca tatgttgtgt tttacagtat tatgtagtct gttttttatg
      121 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt caactttttt
      181 gtacaaactt gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc
      241 gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac
      301 aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat
      361 ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg
      421 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg
      481 ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt
      541 actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct
      601 attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc
      661 ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta
      721 tgaactaatg accccgtaat tgattactat taataactat caacttttct atacaaagtt
      781 ggcattataa aaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagt
//
