LOCUS       sequence-161318-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  red fluorophore without stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: pME-mKate2 no stop
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             98..793
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg ctccaccatg gtgagcgagc tgattaagga
      121 gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac
      181 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggcggtcga
      241 gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa
      301 aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct tccccgaggg
      361 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
      421 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc
      481 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggcctcca ccgagaccct
      541 gtaccccgct gacggcggcc tggaaggcag agccgacatg gccctgaagc tcgtgggcgg
      601 gggccacctg atctgcaact tgaagaccac atacagatcc aagaaacccg ctaagaacct
      661 caagatgccc ggcgtctact atgtggacag aagactggaa agaatcaagg aggccgacaa
      721 agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa
      781 actggggcac agatacccag ctttcttgta caa
//
