LOCUS       sequence-161331-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  nuclear localization signal fused to N-terminus of GFP (nlsGFP) 
            without stop codon, followed by P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: pME-nlsGFP-P2A
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             107..127
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(173..175,176..178,179..691)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(218..239)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(479..498)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     826..847
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctgccacc atggctccaa agaagaagcg
      121 taaggtaaat cgatcgaatt caaggcctct cgagcctcta gaaggtggcg gaatggtgag
      181 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      241 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      301 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      361 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      421 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      481 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      541 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      601 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      661 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      721 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      781 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      841 gttcgtgacc gccgccgg
//
