LOCUS       sequence-161333-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Calreticulin mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Del Cid N, Jeffery E, Rizvi SM, Stamper E, Peters LR, Brown WC, 
            Provoda C, Raghavan M
  TITLE     Modes of calreticulin recruitment to the major histocompatibility 
            complex class I assembly pathway.
  JOURNAL   J Biol Chem. 2010 Feb 12;285(7):4520-35. doi: 
            10.1074/jbc.M109.085407. Epub 2009 Dec 3.
  PUBMED    19959473
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.085407"; journalName: "J Biol Chem"; date: 
            "2010-02-12- 12"; volume: "285"; issue: "7"; pages: "4520-35"
COMMENT     Sequence Label: pmcsg7-mCRT(deltaC)
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat
       61 catcattctt ctggtgtaga tctgggtacc gagaacctgt acttccaatc caatgctgcc
      121 gcagaccctg ccatctattt caaagagcag ttcttggacg gagatgcctg gaccaaccgc
      181 tgggtcgaat ccaaacataa gtccgatttt ggcaaatttg tcctcagttc tggcaaattt
      241 tacggggacc tggagaagga taaagggctg cagacaagcc aagatgcccg attttacgca
      301 ctgtccgcca aattcgaacc cttcagcaat aagggccaga cactggtggt acagttcacg
      361 gtgaagcatg agcagaatat cgactgtggg ggcggctacg tgaagctgtt tccgagtggc
      421 ttggaccaga aggacatgca tggagactca gaatataaca tcatgtttgg tccggacatc
      481 tgcggtcctg gcaccaagaa ggttcatgtc atctttaact acaagggcaa gaatgtgctg
      541 atcaacaagg atatccggtg taaggatgat gaattcacac acctatacac actgattgtg
      601 cggccagaca acacctatga ggtgaaaatt gacaacagcc aggtggagtc aggctccttg
      661 gaggatgatt gggactttct gccacccaag aagataaagg accctgatgc tgccaagccg
      721 gaagactggg atgaacgagc caagatcgat gaccccacag attccaagcc tgaggactgg
      781 gacaagccag agcacatccc tgaccctgat gctaagaagc ctgaggactg ggatgaagag
      841 atggatggag agtgggaacc accagtgatt caaaatcctg aatacaaggg cgagtggaaa
      901 ccacgtcaaa ttgacaaccc agattacaag ggtacctgga tacacccaga aattgacaac
      961 cctgaatact cccccgatgc aaatatctat gc
//
