LOCUS       sequence-161334-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector for IAA15 with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA15_no stop
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..101
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(645..744)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(761..780)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(762..780)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(785..801)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(785..801)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca
       61 atgctttttt ataatgccaa ctttgtacaa aaaagcaggc ttcaaaatgt caccggagga
      121 atacgttagg gtttggccgg attccggtga tcttggagga actgagctga ccctagcttt
      181 gcctggcact ccgacaaatg catcggaagg tccaaaaaag ttcgggaaca aacgtagatt
      241 cctcgagacc gttgatttaa aactcgggga agcacacgag aacaattata tctcaagcat
      301 ggtcactaat gaccagttgg tgggctggcc gccggtagcg acagcgagga aaacagtgag
      361 gcggaaatac gtgaaagtgg cgttggatgg tgcggcctat ctcagaaagg ttgatcttgg
      421 gatgtacgat tgctatggac agcttttcac cgctctagaa aacatgtttc aggggataat
      481 aacaatatgt agagtgacgg agttggagag gaagggagag tttgttgcta cttacgagga
      541 taaggacggt gacttgatgt tagtcggaga tgtgccgtgg atgatgtttg tggaatcttg
      601 caaacgtatg aggttaatga aaaccggaga tgctattgga ttacacccag ctttcttgta
      661 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
      721 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
      781 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
      841 tgcacaagat aaaa
//
