LOCUS       sequence-161342-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector for IAA19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA19
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..101
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(705..804)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(821..840)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(822..840)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(845..861)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(845..861)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca
       61 atgctttttt ataatgccaa ctttgtacaa aaaagcaggc ttcaaaatgg agaaggaagg
      121 actcgggctt gagataacgg agctgagatt ggggcttccg gggagagatg tggcagagaa
      181 gatgatgaag aagagagctt tcacggagat gaatatgacg tcgtcgggta gtaatagtga
      241 tcaatgtgaa agcggcgtcg tttcatctgg tggtgacgct gagaaggtta atgattcgcc
      301 ggcggcgaaa agccaggtgg tggggtggcc accggtttgt tcttaccgga agaaaaacag
      361 ctgtaaggaa gcttcgacca cgaaagtggg gttagggtat gtgaaagtga gcatggatgg
      421 tgtgccttat ttgaggaaga tggatcttgg ttcgagccaa ggctatgatg atctagcctt
      481 tgctcttgat aagctcttcg gtttccgtgg catcggtgtg gccttgaaag atggtgacaa
      541 ctgcgaatac gttaccatat acgaagacaa agatggagac tggatgctcg ccggtgatgt
      601 accttggggg atgtttctag agtcatgcaa gaggttgaga ataatgaaaa gatcggatgc
      661 taccgggttt gggctgcagc ctagaggagt agacgagtga taacacccag ctttcttgta
      721 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
      781 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
      841 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
      901 tgcacaagat aaaa
//
