LOCUS       sequence-161346-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector for IAA2 with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA2_no stop
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(627..726)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(743..762)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(744..762)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(767..783)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(767..783)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg
       61 cttttttata atgccaactt tgtacaaaaa agcaggcttc aaaatggcgt acgagaaagt
      121 caacgagctt aaccttaagg acacagagct atgtcttgga ttacccggaa gaacagagaa
      181 gatcaaagaa gaacaagagg tttcttgcgt taaaagtaac aacaagcgtc tatttgagga
      241 aactcgtgat gaagaagaat ctacacctcc taccaaaact caaatcgttg gttggccacc
      301 agtgagatct tcccgtaaga acaacaacag tgtgagctac gtgaaagtga gtatggacgg
      361 agctccttac cttcgcaaga tcgatctcaa gacatacaaa aactaccccg agcttctcaa
      421 agcgttagag aatatgttca aagtcatgat tggtgaatat tgtgagagag aaggatacaa
      481 aggatctgga tttgtaccaa catacgaaga taaagatggt gactggatgt tggttggtga
      541 tgttccatgg gacatgttct cttcttcttg taagagactc agaatcatga agggatccga
      601 cgctcctgct ctagactctt ccttacaccc agctttcttg tacaaagttg gcattataag
      661 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
      721 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
      781 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaa
//
