LOCUS       sequence-161352-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Calreticulin mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Wijeyesakere SJ, Gafni AA, Raghavan M
  TITLE     Calreticulin is a thermostable protein with distinct structural 
            responses to different divalent cation environments.
  JOURNAL   J Biol Chem. 2011 Mar 18;286(11):8771-85. doi: 
            10.1074/jbc.M110.169193. Epub 2010 Dec 22.
  PUBMED    21177861
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.169193"; journalName: "J Biol Chem"; date: 
            "2011-03-18- 18"; volume: "286"; issue: "11"; pages: "8771-85"
COMMENT     Sequence Label: pmcsg7-mCRT(1-351)
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccaatgctgc cgcagaccct
      121 gccatctatt tcaaagagca gttcttggac ggagatgcct ggaccaaccg ctgggtcgaa
      181 tccaaacata agtccgattt tggcaaattt gtcctcagtt ctggcaaatt ttacggggac
      241 ctggagaagg ataaagggct gcagacaagc caagatgccc gattttacgc actgtccgcc
      301 aaattcgaac ccttcagcaa taagggccag acactggtgg tacagttcac ggtgaagcat
      361 gagcagaata tcgactgtgg gggcggctac gtgaagctgt ttccgagtgg cttggaccag
      421 aaggacatgc atggagactc agaatataac atcatgtttg gtccggacat ctgcggtcct
      481 ggcaccaaga aggttcatgt catctttaac tacaagggca agaatgtgct gatcaacaag
      541 gatatccggt gtaaggatga tgaattcaca cacctataca cactgattgt gcggccagac
      601 aacacctatg aggtgaaaat tgacaacagc caggtggagt caggctcctt ggaggatgat
      661 tgggactttc tgccacccaa gaagataaag gaccctgatg ctgccaagcc ggaagactgg
      721 gatgaacgag ccaagatcga tgaccccaca gattccaagc ctgaggactg ggacaagcca
      781 gagcacatcc ctgaccctga tgctaagaag cctgaggact gggatgaaga gatggatgga
      841 gagtgggaac caccagtgat tcaaaatcct gaatacaagg gcgagtggaa accacgtcaa
      901 attgacaacc cagattacaa gggtacctgg atacacccag aaattgacaa ccctgaatac
      961 tcccccgatg caaatatct
//
