LOCUS       sequence-161353-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector for IAA28 with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA28_no stop
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..101
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(633..732)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(749..768)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(750..768)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(773..789)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(773..789)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca
       61 atgctttttt ataatgccaa ctttgtacaa aaaagcaggc ttcaaaatgg aagaagaaaa
      121 gagattggag ctaaggctag ctcctccttg tcaccaattc acttccaaca acaacatcaa
      181 tggatctaaa caaaaaagct cgaccaaaga aacatcattc ctttccaata acagggttga
      241 ggtagctcca gtggtgggat ggccgccggt gagatcatcc cggagaaacc taacggcaca
      301 actaaaggag gagatgaaga agaaggagag tgatgaagag aaggaattgt acgttaagat
      361 caacatggaa ggagttccaa taggaagaaa agtcaacctt tcagcttata acaactacca
      421 acagctttca catgccgttg accaactctt ctctaagaaa gattcgtggg atctaaacag
      481 acaatacact ttggtctacg aagacactga aggagataaa gttctggtcg gggatgttcc
      541 ttgggagatg tttgtatcta ctgtaaagag gttgcatgtt ttaaagacct cccacgcctt
      601 ctcactctca cctagaaaac atggcaagga acacccagct ttcttgtaca aagttggcat
      661 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
      721 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
      781 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
      841 aa
//
