LOCUS       sequence-161358-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Calreticulin mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Wijeyesakere SJ, Gafni AA, Raghavan M
  TITLE     Calreticulin is a thermostable protein with distinct structural 
            responses to different divalent cation environments.
  JOURNAL   J Biol Chem. 2011 Mar 18;286(11):8771-85. doi: 
            10.1074/jbc.M110.169193. Epub 2010 Dec 22.
  PUBMED    21177861
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.169193"; journalName: "J Biol Chem"; date: 
            "2011-03-18- 18"; volume: "286"; issue: "11"; pages: "8771-85"
COMMENT     Sequence Label: pmcsg7-mCRT(1-351)
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tctttcgggc tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg
       61 ccgcaagctt gtcgacggag ctcgaattcg gatccgttat ccacttccaa tgttacagct
      121 catccttggc ttggccaggg gattcttcct catcttcctc cttctcatct tcttcatcct
      181 catcttcatc tctgtcatca tcatcctctt tatcctcagc ttcttcttcc tctttacgct
      241 tcttgtcctc ttcttcttac ttaagcctct gctcctcatc ctgcttgtcc ttcatctgct
      301 tctctgcagc cttggtaaca ccccacgtct cattgccaaa ctcctctgca taggcctcat
      361 cattggtgat gaggaaattg tcaaagattg tcccggactt gacctgccag agatctaggc
      421 ccagtacagc aaaactatca taggcataga tatttgcatc gggggagtat tcagggttgt
      481 caatttctgg gtgtatccag gtacccttgt aatctgggtt gtcaatttga cgtggtttcc
      541 actcgccctt gtattcagga ttttgaatca ctggtggttc ccactctcca tccatctctt
      601 catcccagtc ctcaggcttc ttagcatcag ggtcagggat gtgctctggc ttgtcccagt
      661 cctcaggctt ggaatctgtg gggtcatcga tcttggctcg ttcatcccag tcttccggct
      721 tggcagcatc agggtccttt atcttcttgg gtggcagaaa gtcccaatca tcctccaagg
      781 agcctgactc cacctggctg ttgtcaattt tcacctcata ggtgttgtct ggccgcacaa
      841 tcagtgtgta taggtgtgtg aattcatcat ccttacaccg gatatccttg ttgatcagca
      901 cattcttgcc cttgtagtta aagatgacat gaaccttctt ggtgccagga ccgcagatgt
      961 ccggaccaaa catgatgtta tattctgagt c
//
