LOCUS       sequence-161362-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CMVmin-promoted GFPmem without pA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-CMVmin:GFPmem no-pA
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..128
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(249..270)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     598..617
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcgcggccgc gttaacgcta ccatggagct ccaaataatg attttatttt gactgatagt
       61 gacctgttcg ttgcaacaaa ttgataagca atgctttttt ataatgccaa ctttgtataa
      121 taaagttgtc aggagagcac acacttgcag ctcatgcagc cggggccact ctcatcagga
      181 gggttcagct tagatcttcc tcctcccttg tacagctcgt ccatgccgag agtgatcccg
      241 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      301 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      361 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
      421 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
      481 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      541 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      601 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      661 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
      721 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtg
//
