LOCUS       sequence-161363-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector for IAA31.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA31
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..101
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(585..684)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(701..720)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(702..720)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(725..741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(725..741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca
       61 atgctttttt ataatgccaa ctttgtacaa aaaagcaggc ttcaaaatgg aggtctctaa
      121 ctcttgttct tcattttctt catcctctgt cgacagtact aaaccttctc cttctgaatc
      181 ttctgttaat ctctccctta gtctcacatt tccttctact tctccacaaa gagaagcaag
      241 acaagattgg ccaccgataa agtctagatt aagagataca ctaaagggtc gtcgtcttct
      301 tcgtcgtggt gatgacactt ctctctttgt taaggtttat atggaaggtg ttcccattgg
      361 aagaaaactc gacctttgcg tattctcagg ctacgagagt ctattagaaa atctctctca
      421 catgttcgat acttcaatca tctgcggtaa tcgagatcga aaacatcatg ttttgacata
      481 tgaagacaag gatggagatt ggatgatggt cggagatatt ccatgggata tgtttcttga
      541 aaccgtgaga agactaaaga tcacgagacc ggagaggtat taacacccag ctttcttgta
      601 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
      661 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
      721 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
      781 tgcacaagat aaaa
//
