LOCUS       sequence-161376-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  nlsGFP with pA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-nlsGFP pA
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..128
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    130..354
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     343..360
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        380..398
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     380..397
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(478..499)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     827..846
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcgcggccgc gttaacgcta ccatggagct ccaaataatg attttatttt gactgatagt
       61 gacctgttcg ttgcaacaaa ttgataagca atgctttttt ataatgccaa ctttgtataa
      121 taaagttggc catagagccc accgcatccc cagcatgcct gctattgtct tcccaatcct
      181 cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat
      241 gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca
      301 aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg
      361 ctgatcagcg agctctagca tttaggtgac actatagaat agggccctct agatgcatgc
      421 tcgagcggcc gcttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
      481 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      541 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      601 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      661 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      721 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      781 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
      841 gtcgtcctt
//
