LOCUS       sequence-161389-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector for IAA4 with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA4_no stop
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..101
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(666..765)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(782..801)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(783..801)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(806..822)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(806..822)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca
       61 atgctttttt ataatgccaa ctttgtacaa aaaagcaggc ttcaaaatgg aaaaagttga
      121 tgtttatgat gagcttgtta acctaaaggc aacagagctg agattgggat taccagggac
      181 agaagaaact gtttcttgtg gaaaaagcaa caaaagagtt ttacctgaag ctactgagaa
      241 agagattgaa tccactggaa aaactgaaac cgcttctcct ccaaaggctc agattgttgg
      301 atggccacca gttagatctt acagaaagaa caatgttcag acaaagaaga gtgaatctga
      361 aggtcaagga aactatgtga aagtaagtat ggatggtgct ccatatctaa ggaagataga
      421 tctaacgatg tataaacaat atccagagtt gatgaaatca cttgaaaaca tgtttaaatt
      481 ctctgtggga gaatattttg agagagaagg atataaaggc tcagactttg tacctactta
      541 tgaagacaaa gatggtgatt ggatgcttgt tggtgatgtt ccttgggaga tgtttgtttc
      601 gtcttgtaag aggctaagga tcatgaaagg atctgaagtt aaaggtttag gttgtggtgg
      661 tcttcaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
      721 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
      781 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
      841 tcaaaa
//
