LOCUS       sequence-161392-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector for IAA5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA5
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(589..688)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(705..724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(706..724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(729..745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(729..745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagcaggct tcaaaatggc gaatgagagt aataatcttg
      121 gactcgaaat caccgaacta cggctaggtc ttcccggaga tatcgtcgtc tccggtgagt
      181 ccatctccgg gaagaagagg gcttctccgg aagtagagat tgatttgaaa tgtgaaccgg
      241 cgaaaaagag tcaagttgtg ggttggccac cggtttgttc gtaccggaga aagaacagtc
      301 tcgaacggac caaaagttcg tacgtgaaag tgagtgtaga tggagctgca tttttaagga
      361 agattgattt ggaaatgtac aaatgttacc aagatcttgc ttccgctctg caaattctgt
      421 tcggatgcta tatcaatttt gatgatacgt tgaaggaaag tgaatgtgta ccaatatatg
      481 aagacaaaga tggagattgg atgcttgctg gagatgttcc ttgggaaatg ttccttggat
      541 cgtgcaagag gttaaggatt atgaagagat catgtaacag aggatgacac ccagctttct
      601 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
      661 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
      721 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
      781 acattgcaca agataaaa
//
