LOCUS       sequence-161397-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CMVmin-promoted nlsGFP with pA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-CMVmin:nlsGFP pA
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(38..161)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(187..206)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        378..757
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 attctacagg tcactaatac catctaagta gttggttcat agtgactgca tatgttgtgt
       61 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
      121 atcattttac gtttctcgtt caactttctt gtacaaagtg gccgatcccc tatggtgcac
      181 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
      241 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
      301 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
      361 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
      421 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      481 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      541 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      601 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      661 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      721 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      781 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
//
