LOCUS       sequence-161400-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector for IAA6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA6
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..101
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(678..777)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(794..813)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(795..813)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(818..834)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(818..834)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca
       61 atgctttttt ataatgccaa ctttgtacaa aaaagcaggc ttcaaaatgg caaaggaagg
      121 tctagcactc gagatcacag agcttcgatt gggtcttcca ggagataatt atagcgaaat
      181 atcagtatgc ggatcgagta agaagaagaa gagggtgctc tcggatatga tgacctcatc
      241 agcgttagat actgagaatg aaaacagcgt cgtttcatca gttgaagatg aatcactgcc
      301 ggttgtgaag agtcaagcgg tgggatggcc acctgtgtgt tcttacagga gaaagaagaa
      361 caatgaggaa gcatcgaaag ctataggcta cgtgaaagtg agcatggatg gtgtgccata
      421 catgaggaag attgaccttg gttcgagcaa cagttatatt aatctagtca cggttcttga
      481 gaatctcttc ggctgtcttg gcataggagt ggcgaaggag ggtaagaagt gtgaatacat
      541 tattatatac gaagacaagg atagagactg gatgctcgtc ggagatgtac cttggcagat
      601 gtttaaagaa tcatgcaaga ggctgaggat cgtgaagaga tcagatgcaa ctggttttgg
      661 tctccagcaa gattaacacc cagctttctt gtacaaagtt ggcattataa gaaagcattg
      721 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
      781 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
      841 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaa
//
