LOCUS       sequence-161410-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of GATA3 fused to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  TITLE     Engel Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV-GATA3gfp
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(136..849,850..852,853..855))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(139..849,850..852,853..855))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(181..202)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     530..549
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     789..810
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
       61 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct
      121 agagtcgcgg ccgctttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt
      181 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga
      241 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
      301 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
      361 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta
      421 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
      481 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
      541 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
      601 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt
      661 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
      721 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg
      781 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
      841 gcccttgctc accatggcgg tgaccatgct ggaagggtgg tgaggtccga aggagaggcc
      901 ggagggcgga tgcatgggcg tcggtgtggt cagcatgtgg ctggagtggc tgaagggaga
      961 gatgtggctc agggatgaca tgtgtctgga gagagcggcc gggttgaagg agctgctctt
     1021 ggggaagtcc tccagcgcgt catgcacctt tttgcacttt ttcgatttgc tagacatctt
     1081 ccggtttc
//
