LOCUS       sequence-161414-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector for IAA7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA7
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(835..934)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(951..970)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(952..970)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
       61 tttttataat gccaactttg tacaaaaaag caggcttcaa aatgatcggc caacttatga
      121 acctcaaggc cacggagctc tgtctcggcc tccccggcgg cgctgaagca gttgagagtc
      181 ctgccaaatc ggcggtggga agcaagagag gcttctccga aaccgttgat ctcatgctca
      241 atcttcaatc taacaaagaa ggctccgttg atctcaaaaa cgtttctgct gttcccaagg
      301 agaagactac ccttaaagat ccttctaagc ctcctgctaa agcacaagtg gtgggatggc
      361 cacctgtgag gaactacagg aagaacatga tgactcagca gaagaccagt agtggtgcgg
      421 aggaggccag cagtgagaag gccgggaact ttggtggagg agcagccgga gccggcttgg
      481 tgaaggtctc catggacggt gctccatatc tgaggaaagt tgacctcaag atgtacaaaa
      541 gctaccagga tctttctgat gcattggcca aaatgttcag ctcctttact atgggaaact
      601 atggagcaca aggaatgata gatttcatga acgagagcaa gctaatgaat ctgctgaata
      661 gctctgagta tgtgccaagc tacgaggaca aagatggtga ctggatgctc gttggcgatg
      721 tcccatggga aatgtttgtc gagtcttgca aacgtttgcg cattatgaag ggatctgaag
      781 cagttggact tgctccgaga gcaatggaga agtactgcaa gaacagatct tgacacccag
      841 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
      901 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
      961 agtcgtatta catgg
//
