LOCUS       sequence-161417-        3892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CCND1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_CCND1_p.E172K
FEATURES             Location/Qualifiers
     source          1..3892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(85..104)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(86..104)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(109..125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(109..125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             556..1347
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1440..2028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1929..1948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2182..2199
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2358..2385)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2477..2563)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2612..2634
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2627..2643
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2659..2758
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     2760..2776
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      3573..3582
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(join(3861..3892,1..68))
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
       61 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
      121 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
      181 atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
      241 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
      301 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
      361 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
      421 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      481 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      541 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      601 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      661 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
      721 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      781 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      841 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      901 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      961 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     1021 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     1081 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1141 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1201 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1261 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     1321 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     1381 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1441 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1501 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1561 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1621 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1681 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1741 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1801 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1861 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1921 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1981 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2041 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2101 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2161 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     2221 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     2281 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     2341 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     2401 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     2461 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     2521 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     2581 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     2641 agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac ctgttcgttg
     2701 caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa agcaggcttg
     2761 taaaacgacg gccagtaact ataacggtcc taaggtagcg aggcctgggt ggcgaattcg
     2821 gcacgaggca gtagcagcga gcagcagagt ccgcacgctc cggcgagggg cagaagagcg
     2881 cgagggagcg cggggcagca gaagcgagag ccgagcgcgg acccagccag gacccacagc
     2941 cctccccagc tgcccaggaa gagccccagc catggaacac cagctcctgt gctgcgaagt
     3001 ggaaaccatc cgccgcgcgt accccgatgc caacctcctc aacgaccggg tgctgcgggc
     3061 catgctgaag gcggaggaga cctgcgcgcc ctcggtgtcc tacttcaaat gtgtgcagaa
     3121 ggaggtcctg ccgtccatgc ggaagatcgt cgccacctgg atgctggagg tctgcgagga
     3181 acagaagtgc gaggaggagg tcttcccgct ggccatgaac tacctggacc gcttcctgtc
     3241 gctggagccc gtgaaaaaga gccgcctgca gctgctgggg gccacttgca tgttcgtggc
     3301 ctctaagatg aaggagacca tccccctgac ggccgagaag ctgtgcatct acaccgacaa
     3361 ctccatccgg cccgaggagc tgctgcaaat ggagctgctc ctggtgaaca agctcaagtg
     3421 gaacctggcc gcaatgaccc cgcacgattt cattgaacac ttcctctcca aaatgccaga
     3481 ggctaaggag aacaaacaga tcatccgcaa acacgcgcag accttcgttg ccctctgtgc
     3541 cacagatgtg aagttcattt ccaatccgcc ctccatggtg gcagcgggga gcgtggtggc
     3601 cgcagtgcaa ggcctgaacc tgaggagccc caacaacttc ctgtcctact accgcctcac
     3661 acgcttcctc tccagagtga tcaagtgtga cccggactgc ctccgggcct gccaggagca
     3721 gatcgaagcc ctgctggagt caagcctgcg ccaggcccag cagaacatgg accccaaggc
     3781 cgccgaggag gaggaagagg aggaggagga ggtggacctg gcttgcacac ccaccgacgt
     3841 gcgggacgtg gacatctgat tgccaacttt cttgtacaaa gttggcatta ta
//
