LOCUS       sequence-161432-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  P2A followed by multiple cloning site without pA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-P2A-MCS no-pA
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..169)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             193..249
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    250..357
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     266..282
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     267..283
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(317..333)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(317..333)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(365..460)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(497..516)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(498..516)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(521..537)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(521..537)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(639..658)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(717..736)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gagctcgaat tctacaggtc actaatacca tctaagtagt tggttcatag tgactgcata
       61 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
      121 atatttatat cattttacgt ttctcgttca actttcttgt acaaagtgga agaattgggt
      181 accggatccg gagccacgaa cttctctctg ttaaagcaag caggagacgt ggaagaaaac
      241 cccggtcctg gtaccgggcc ccccctcgag gtcgacggta tcgataagct tgatatcgaa
      301 ttcctgcagc ccgggggatc cactagttct agagcggccg ccaccgcggt ggagctccag
      361 cttgcaactt tattatacaa agttggcatt ataaaaaagc attgcttatc aatttgttgc
      421 aacgaacagg tcactatcag tcaaaataaa atcattattt ggagctccat ggtagcgtta
      481 acgcggccgc gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
      541 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
      601 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
      661 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
      721 taaatgggct cgcgataatg tcgg
//
