LOCUS       sequence-161438-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  P2A followed by mKate2 without pA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-P2A-mKate2 no-pA
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..169)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             193..249
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gagctcgaat tctacaggtc actaatacca tctaagtagt tggttcatag tgactgcata
       61 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
      121 atatttatat cattttacgt ttctcgttca actttcttgt acaaagtgga agaattgggt
      181 accggatccg gagccacgaa cttctctctg ttaaagcaag caggagacgt ggaagaaaac
      241 cccggtcctg gtaccgggcc ccccctcgag atggtgagcg agctgattaa ggagaacatg
      301 cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag
      361 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggcggt cgagggcggc
      421 cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag caaaaccttc
      481 atcaaccaca cccagggcat ccccgacttc tttaagcagt ccttccccga gggcttcaca
      541 tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc
      601 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac
      661 ggccctgtga tgcagaagaa aacactcggc tgggaggcct ccaccgagac cctgtacccc
      721 gctgacggcg gcctggaagg cagagccgac atggccctga agctcgtggg cgggggccac
//
