LOCUS       sequence-161444-         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for conjugational transfer into cyanobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Kukil K, Lindberg P
  TITLE     Expression of phenylalanine ammonia lyases in Synechocystis sp. PCC 
            6803 and subsequent improvements of sustainable production of 
            phenylpropanoids.
  JOURNAL   Microb Cell Fact. 2022 Jan 10;21(1):8. doi: 
            10.1186/s12934-021-01735-8.
  PUBMED    35012528
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2022-01-10"; volume: "21(1):8. doi"; pages: " 
            10.1186/s12934-021-01735-8"
COMMENT     Sequence Label: pEEK1
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    130..146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(154..160,161..177,178..183))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    218..237
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     terminator      248..291
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(295..313)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        381..485
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
       61 aatataaatg tcaggctccc ttatacacag ccagtctcta gaacctccac tactcgagta
      121 tgtgtgaaat tgttatccgc tcacaattcc acacattata cgagccggat gattaattgt
      181 caacagctca tttcagaata tttgccagaa ccgttcgaat tgtgagcgct cacaattcgg
      241 aattccagaa atcatcctta gcgaaagcta aggatttttt ttatctgaaa ttctgcctcg
      301 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      361 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      421 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      481 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      541 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      601 gtgcacgagt gggttacatc gaact
//
